Plasmodium falciparum lactate dehydrogenase (pL2) Aptamer Details
ID# 7696
Structure
Chemistry
DNA
Target
Plasmodium falciparum lactate dehydrogenase (PfLDH)
Category
Protein
Affinity (Kd)
49.6 nM
Length
38
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdApdApdCpdTpdCpdApdTpdTpdGpdGpdCpdTpdGpdGpdApdGpdGpdCpdGpdGpdCpdApdGpdTpdApdCpdCpdGpdCpdTpdTpdGpdApdGpdTpdTpdCp-3'
Molecular Weight: 11750.66
Extinction Coefficient: 358500
Binding Conditions / Buffer
Binding Buffer: 20mM Trisƒ??HCl pH 8.0, 50mM NaCl, 5mM KCl, and 5mM MgCl2)
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Seonghwan, L. (2012). A highly sensitive aptasensor towards plasmodium lactate dehydrogenase for the diagnosis of malaria. Biosensors and Bioelectronics, 35(2012), 291-296.
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