Phospholamban (Apt-9) Aptamer Details
ID# 7584
Structure
Chemistry
DNA
Target
Phospholamban
Category
Protein
Affinity (Kd)
20 nM
Length
40
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dTpdTpdGpdGpdGpdGpdApdGpdGpdGpdGpdCpdApdCpdTpdGpdGpdGpdCpdApdGpdTpdGpdTpdApdApdTpdTpdTpdApdCpdGpdApdApdApdGpdCpdGpdApdGp-3'
Molecular Weight: 12546.21
Extinction Coefficient: 400500
Binding Conditions / Buffer
10 mL of PBS (0..5% Tween 20) containing bead-bound PDZ-Myc-His6 (5 uL) and 1 ug/mL BSA was incubated for 30 minutes with library.
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Tanaka et al. "In Vitro Selection and Characterization of DNA Aptamers Specific for Phospholamban." The Journal of Pharmacology and Experimental Therapeutics, 329(2009): 57-63.
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