Phorate (SS4-54) Aptamer Details
ID# 7681
Structure
Chemistry
DNA
Target
Phorate
Category
Small Organic
Affinity (Kd)
1.43 µM
Length
54
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdApdGpdCpdTpdTpdTpdTpdTpdTpdGpdApdCpdTpdGpdApdCpdTpdGpdCpdApdGpdCpdGpdApdTpdTpdCpdTpdTpdGpdApdTpdCpdGpdCpdCpdApdCpdGpdGpdTpdCpdTpdGpdGpdApdApdApdApdApdGpdApdGp-3'
Molecular Weight: 16675.9
Extinction Coefficient: 517600
Binding Conditions / Buffer
Selection buffer used in binding affinity assays: 300mM NaCl, 50 mM KCl, 10 mM MgCl2, 50 mM Tris/HCl, and pH 8.3
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Wang, L, et al. (2012). Selection of DNA aptamers that bind four organophosphorus pesticides. Biotechnol Lett, 2012(34), 869-874. doi: 10.1007/s10529-012-0850-6
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