Phenylalanine Aptamer Details
ID# 7559
Structure
Chemistry
RNA
Target
Phenylalanine
Category
Small Organic
Affinity (Kd)
34 µM
Length
80
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rAprUprUprGprGprAprUprCprGprGprUprAprGprUprAprUprUprUprAprGprGprGprUprGprAprGprAprCprAprCprUprUprCprAprUprGprCprCprUprUprUprGprUprUprGprCprAprGprGprCprUprGprGprGprGprUprGprAprAprGprGprCprGprCprUprAprCprAprUprGprGprCprGprUprCprUprGprAprAprAp-3'
Molecular Weight: 25864.53
Extinction Coefficient: 800800
Binding Conditions / Buffer
Selection Buffer (50 mM Hepes (pH 7.0), 0.3 M NaCl, 5 mM CaCl2, and 5 mM MgCl2)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Illangasekare and Marus. "Phenylalanine-Binding RNAs and Genetic Code Evolution." Journal of Molecular Evolution, 54(2002): 298-311.
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