PFOA Aptamer Aptamer Details
ID# 8177
Structure
Chemistry
DNA
Target
Polyfluoroalkyl acid (Perfluorooctanoic acid, PFOA)
Category
Small Organic
Affinity (Kd)
5.5 nM
Length
30
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdApdApdTpdCpdGpdTpdGpdGpdGpdTpdGpdGpdTpdApdGpdGpdGpdTpdApdGpdGpdGpdGpdApdTpdGpdCpdAp-3'
Molecular Weight: 9488.22
Extinction Coefficient: 308100
Binding Conditions / Buffer
pH 7.5 buffer (20 mM HEPES, 1 M NaCl, 10 mM MgCl2, and 5 mM KCl)
Binding Temp: 23°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Park, J., Yang, K.-A., Choi, Y., & Choe, J. K. (2022). Novel ssdna aptamer-based fluorescence sensor for perfluorooctanoic acid detection in water. Environment International, 158, 107000. https://doi.org/10.1016/j.envint.2021.107000
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