Peroxidase DNAzyme ([B7]-3-0) Aptamer Details

ID# 7834

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Structure

Chemistry

DNA

Target

Hemin

Sequence (3-char table code)

G A C U/T

5'-dApdTpdTpdGpdGpdGpdApdGpdGpdGpdApdTpdTpdGpdGpdGpdTpdGpdGpdGp-3'

Molecular Weight: 6349.19

Extinction Coefficient: 203600

Binding Conditions / Buffer

Binding buffer: 20 mM Tris-AcOH (pH 8.0), 100 mM NaCl, 200 mM KCl, 5 mM MgCl2, 0.5% Triton X-100, and 5% DMSO. Peroxidase activity was measured using the ABTS-H2O2 system.

Binding Temp: RT°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Zhu L, et al. (2012) In vitro selection of highly efficient G-quadruplex-based DNAzymes. Anal. Chem. 84: 8383-8390.

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