Penicillin Binding Protein 2a (PBP2a) (Aptamer 1) Aptamer Details

ID# 10009

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Structure

Chemistry

DNA

Target

Penicillin-binding protein 2a (PBP2a)

Sequence (3-char table code)

G A C U/T

5'-dGpdGpdGpdApdGpdCpdTpdCpdApdGpdApdApdTpdApdApdApdCpdGpdCpdTpdCpdApdApdTpdApdCpdTpdApdTpdCpdGpdCpdGpdGpdApdGpdApdCpdApdGpdCpdGpdCpdGpdGpdGpdApdGpdGpdCpdApdCpdCpdGpdGpdGpdGpdApdGpdApdTpdCpdCpdGpdGpdGpdCpdCpdTpdCpdApdTpdGpdTpdCpdGpdApdAp-3'

Molecular Weight: 24229.7 g/mole

Extinction Coefficient: 767200

Binding Conditions / Buffer

20 mmol/L HEPES buffer (N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid) at pH 7.35, 120 mmol/L NaCl, 1 mmol/L MgCl2, 1 mmol/L CaCl2, and 5 mmol/L KCl

Binding Temp: 37°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Ning Y, Wang X, Chen P, Liu S, Hu J, Xiao R, Li L, Lu F. Targeted inhibition of methicillin-resistant Staphylococcus aureus biofilm formation by a graphene oxide-loaded aptamer/berberine bifunctional complex. Drug Deliv. 2022 Dec;29(1):1675-1683. doi: 10.1080/10717544.2022.2079768.

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