PCB77 (II) Aptamer Details
ID# 7689
Structure
Chemistry
DNA
Target
3,3,4,4-tetrachlorobiphenyl (PCB77)
Category
Small Organic
Affinity (Kd)
8.32 µM
Length
38
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdApdGpdCpdGpdCpdGpdCpdGpdApdGpdApdCpdTpdApdCpdGpdTpdTpdTpdGpdTpdApdGpdGpdGpdApdTpdApdCpdCpdGpdApdTpdGpdTpdCpdGp-3'
Molecular Weight: 11783.7
Extinction Coefficient: 374200
Binding Conditions / Buffer
Binding Buffer: 100 mM NaCl, 20 mM Trisƒ??HCl [pH 7.6], 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, and 0.02% Tween 20
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Shengmin, X. (2012). Selection of dna aptamers against polychlorinated biphenyls as potential biorecognition elements for environmental analysis. Analytical Biochemistry, 423(2012), 195-201.
Request a Quote
Have your aptamer oligo synthesized by Aptagen.
