PCB72 (Aptamer 9.3) Aptamer Details
ID# 7620
Structure
Chemistry
DNA
Target
Polychlorinated biphenyl 72 (PCB)
Category
Small Organic
Affinity (Kd)
66 nM
Length
80
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdGpdCp dApdGpdCp dApdCpdAp dGpdApdGp dGpdTpdCp dApdGpdAp dTpdGp dCpdApdCp dTpdCpdGp dGpdApdCp dCpdCpdCp dApdTpdTp dCpdTpdCp dCpdTpdTp dCpdCpdAp dTpdCpdCp dCpdTpdCp dApdTpdCp dCpdGpdTp dCpdCpdAp dCp dCpdCpdTp dApdTpdGp dCpdGpdTp dGpdCpdTp dApdCpdCp dGpdTpdGp dApdAp -3'
Molecular Weight: 24336.74
Extinction Coefficient: 735000
Binding Conditions / Buffer
Binding Buffer (100 mM NaCl, 20 mM Trisƒ??HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, pH 7.6)
Binding Temp: r.t.°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Mehta et al. "Selection and characterization of PCB-binding DNA aptamers." Analytical Chemistry 84(2012): 1669-1676.
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