PC12 Cells (Aptamer17a) Aptamer Details

ID# 7618

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Structure

Chemistry

DNA

Target

PC12 Cells

Sequence (3-char table code)

G A C U/T

5'-dGpdGpdTpdTpdGpdGpdApdTpdGpdTpdApdApdGpdGpdTpdTpdGpdGpdApdGpdGpdGpdGpdGpdGp-3'

Molecular Weight: 7954.23

Extinction Coefficient: 255700

Binding Conditions / Buffer

Washed with selection buffer (5 mM MgCl in PBS) from and 1 mg/ml yeast tRNAfor 30 minutes.

Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Liu et al. "Dissection of the Functional Structure of Aptamer17, Which Specifically Recognizes Differential PC12 Cells." Nucleic Acid Therapeutics, 21(2011): 225-229. Wang et al. "Single-stranded DNA Aptamers thatBbind Differentiated but not Parental Cells: Subtractive Systematic Evolution of Ligands by Exponential Enrichment." J. Biotechnol., 102(2003): 15-22.

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