PC 12-31 Aptamer Details
ID# 8009
Structure
Chemistry
DNA
Target
Escherichia Coli (E.Coli)
Category
Other
Affinity (Kd)
87.03 nM
Length
37
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'- dCpdCpdCp dTpdCpdCp dGpdGpdGp dGpdGpdGp dGpdTpdCp dApdTpdCp dGpdGpdGp dApdTpdAp dCpdCpdTp dGpdGpdTp dApdApdGp dGpdApdTp dAp -3'
Molecular Weight: 11471.4
Extinction Coefficient: 358700
Binding Conditions / Buffer
PBS containing 1.4mM MgCl2
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
S. Marton, F. Cleto, M.A. Krieger, J. Cardoso, Isolation of an aptamer that binds specifically to E. coli, PLoS One. 11 (2016) 1–17, https://doi.org/10.1371/journal.
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