Aptagen

Pb(II) Aptamer Details

ID# 7593
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Structure
Pb(II) structure
Chemistry
DNA
Target
Pb(II)
Category
Small Organic
Affinity (Kd)
4 nM
Length
56
Aptazyme
Yes

Sequence (3-char table code)

G A C U/T
5'-dApdCpdTpdCpdApdCpdTpdApdTp-R-N-dGpdGpdApdApdGpdApdGpdApdTpdGpdGpdCpdGpdApdCpdApdTpdCpdTpdCpdTpdTpdCpdTpdCpdCpdGpdApdGpdCpdCpdGpdGpdTpdCpdGpdApdApdApdTpdApdGpdTpdGpdApdGpdTp-3'
Molecular Weight: 17288.28
Extinction Coefficient: 547100

Binding Conditions / Buffer

Buffer A (50 mM HEPES, 500 mM NaCl, 20 uM EDTA (pH 7.0)
Binding Temp:

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Lu et al. "New highly sensitive and selective catalytic DNA biosensors for metal ions." Biosensors and Bioelectronics, 18(2003): 529-540.
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