PB-7S Aptamer Details

ID# 8876

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Structure

Chemistry

DNA

Target

Pb(||)

Sequence (3-char table code)

G A C U/T

5'-dGpdGpdGpdApdCpdGpdApdCpdGpdGpdCpdApdGpdGpdGpdCpdTpdGpdTpdCpdGpdTpdApdCpdGpdGpdTpdTpdTpdGpdTpdCpdGpdApdApdGpdGpdTpdGpdTpdCpdGpdTpdCpdCpdCp-3'

Molecular Weight: 14295.2

Extinction Coefficient: 435000

Binding Conditions / Buffer

SB-buffer (100M Al(NO3)3, 100M Cu(NO3)2, 100M Co(NO3)2, 100M Zn(NO3)2, 100M Ca(NO3)2 and 100M Ba(NO3)2 in SELEX buffer) 200L of SELEX buffer (20 mM Tris-HAc, 10mM NaAc, pH = 7.4) 200L of 100M Pb(NO3)2 in SELEX buffer 200L of SELEX buffer

Binding Temp: 23°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Chen Y., Li H., Gao T., Zhang T., Xua L., Wang B., Wang J., & Pei R. (2018). Selection of DNA aptamers for the development of light-up biosensor to detect Pb(II). Sensors and Actuators B: Chemical, 254, 214-221. http://dx.doi.org/10.1016/j.snb.2017.07.068

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