Pb-14S Aptamer Details
ID# 8881
Structure
Chemistry
DNA
Target
Pb(||)
Category
Other
Affinity (Kd)
0.76 ± 0.18 µM
Length
42
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdApdCpdGpdApdCpdGpdGpdCpdCpdApdGpdTpdApdGpdCpdTpdGpdApdCpdApdTpdCpdApdGpdTpdGpdTpdApdCpdGpdApdTpdCpdTpdApdGpdTpdCpdGpdTpdCp-3'
Molecular Weight: 12939.4
Extinction Coefficient: 408800
Binding Conditions / Buffer
SB-buffer (100M Al(NO3)3, 100M Cu(NO3)2, 100M Co(NO3)2, 100M Zn(NO3)2, 100M Ca(NO3)2 and 100M Ba(NO3)2 in SELEX buffer) 200L of SELEX buffer (20 mM Tris-HAc, 10mM NaAc, pH = 7.4) 200L of 100M Pb(NO3)2 in SELEX buffer 200L of SELEX buffer
Binding Temp: 23°C
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Chen Y., Li H., Gao T., Zhang T., Xua L., Wang B., Wang J., & Pei R. (2018). Selection of DNA aptamers for the development of light-up biosensor to detect Pb(II). Sensors and Actuators B: Chemical, 254, 214-221. http://dx.doi.org/10.1016/j.snb.2017.07.068
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