P2MC Aptamer Details
ID# 8117
Structure
Chemistry
RNA
Target
Doxycycline
Category
Other
Affinity (Kd)
0.780 ± 1.76 nM
Length
100
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdGpdApdGpdApdCpdCpdGpdCpdTpdTpdApdApdGpdCpdTpdTpdApdApdApdGpdGpdApdGpdCpdGpdGpdTpdTpdApdCpdGpdApdApdTpdGpdCpdGpdApdTpdGpdApdCpdTpdTpdApdGpdGpdTpdApdCpdCpdApdTpdTpdGpdCpdApdCpdTpdApdCpdGpdGpdTpdApdCpdCpdTpdApdApdApdCpdApdGpdTpdTpdCpdCpdTpdTpdTpdGpdGpdApdTpdCpdCpdGpdApdApdTpdTpdCpdGpdCpdCpdGpdCp -3'
Molecular Weight: 30882
Extinction Coefficient: 963900
Binding Conditions / Buffer
50 mM Tris-HCl (pH 8.3), 100 mM KCl, and 20 mM MgCl2
Binding Temp: 65°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Tickner, Zachary J et al. “Selection of High-Affinity RNA Aptamers That Distinguish between Doxycycline and Tetracycline.” Biochemistry vol. 59,37 (2020): 3473-3486. doi:10.1021/acs.biochem.0c00586
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