P-Selectin (PF377) Aptamer Details
ID# 7614
Structure
Chemistry
2'-F-RNA
Target
P-Selectin
Category
Protein
Affinity (Kd)
N/A nM
Length
57
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rApfCprGpfCpfUpfCprAprApfCprGprAprGpfCpfCprAprGprGprAprApfCprApfUpfCprGprApfCprGpfUpfCprAprGpfCprAprAprApfCprGpfCprGprAprGpfCprGpfCprAprApfCpfCprAprGpfUprAprApfCprApfCpfCp-3'
Molecular Weight: 18386.21
Extinction Coefficient: 559700
Binding Conditions / Buffer
Washed with PBS and 0.9 mM CaCl2, 0.5 mM MgCl2, 2.67 mM KCl, 1.47 mM KH2PO4, 135 mM NaCl, and 8 mM Na2.
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Burnette et al. "RNA Aptamer Therapy for Vaso-Occlusion in Sickle Cell Disease." Nucleic Acids Therapeutics, 21(2011): 275-283. Jenison et al. "Oligonucleotide Inhibitors of P-Selectin Dependent Neutrophil-Platelet Adhesion." Antisense & Nucleic Acid Development, 8(1998): 265-279.
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