p-Nosyl Protected Alkyl Amino Group (M6b-M14) Aptamer Details
ID# 7824
Structure
Chemistry
DNA
Target
p-nosyl protected alkyl amino groups
Category
Small Organic
Affinity (Kd)
2.27 nM
Length
32
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'- dApdTpdCpdApdTpdGpdGpdTpdGpdGp dGpdTpdApdTpdCpdGpdGpdCpdApdCp dTpdCpdGpdTpdTpdGpdGpdTpdTpdGp dApdTp-3'
Molecular Weight: 9917.5
Extinction Coefficient: 306300
Binding Conditions / Buffer
Optimized Buffer: 10 mM Na2HPO4, 2 mM KH2, 500 mM NaCl, pH 7.4.
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Mei H et al. (2012) Functional-group specific aptamers indirectly recognizing compounds with alkyl amino group. Anal. Chem. 84: 7323 - 7329
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