Oxytetracycline binding aptamer (OTC-4) Aptamer Details

ID# 8002

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Structure

Chemistry

DNA

Target

Oxytetracycline

Sequence (3-char table code)

G A C U/T

5'-dCpdGpdTpdApdCpdGpdGpdApdApdTpdTpdCpdGpdCpdTpdApdGpdCpdCpdGpdApdCpdGpdCpdGpdCpdGpdTpdTpdGpdGpdTpdGpdGpdTpdGpdGpdApdTpdGpdGpdTpdGpdTpdGpdTpdTpdApdCpdApdCpdGpdTpdGpdTpdTpdGpdTpdGpdGpdApdTpdCpdCpdGpdApdGpdCpdTpdCpdCpdApdCpdGpdTpdGp-3'

Molecular Weight: 23601.29

Extinction Coefficient: 791330

Binding Conditions / Buffer

100 mM NaCl, 20 mM Tris–HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02 Tween 20, pH 7.6

Binding Temp: 25°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Niazi, J. H., Lee, S. J., Kim, Y. S., & Gu, M. B. (2008). ssDNA aptamers that selectively bind oxytetracycline. Bioorganic & Medicinal Chemistry, 16(3), 1254–1261. doi:10.1016/j.bmc.2007.10.073

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