OSM (ADR58) Aptamer Details
ID# 7499
Structure
Chemistry
2'-F-RNA
Target
Human Oncostatin M (OSM)
Category
Protein
Affinity (Kd)
7 nM
Length
70
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprGprApfCprGprApfUprGpfCprGprGprApfUpfCprGpfCpfCpfCpfUprGprAprApfCpfCprGprGpfCpfCpfCprAprGpfCprAprGprApfCpfUprGpfCpfUprGprApfCprGprGpfCprApfCprGprApfUpfCpfCprGpfCprApfUpfCprGpfUpfCpfCpfUpfCpfCpfCp-3'
Molecular Weight: 22572.51
Extinction Coefficient: 651800
Binding Conditions / Buffer
Wash Buffer: 0.1% I-block, 0.05% Tween 20 in SCHMK buffer (110 mM NaCl, 1 mM MgCl2, 20 mM HEPES (pH 7.0), 1 mM CaCl2, 5 mM KCl)
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Rhodes, Andrew, et al. "The generation and characterization of antagonist RNA aptamers to human oncostatin M." Journal of Biological Chemistry, 275 (2000): 28555-28561.
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