OS-7.9 Aptamer Aptamer Details
ID# 8190
Structure
Chemistry
DNA
Target
Human Osteosarcoma MG-63
Category
Cells
Affinity (Kd)
12.8 nM
Length
85
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdTpdCpdCpdApdGpdApdGpdTpdGpdApdCpdGpdCpdApdGpdCpdApdGpdGpdGpdCpdApdCpdApdTpdTpdGpdTpdTpdCpdApdCpdApdCpdApdCpdApdGpdApdTpdCpdApdCpdApdTpdTpdApdCpdGpdGpdApdApdApdApdCpdApdCpdApdApdCpdTpdApdCpdApdCpdGpdApdApdApdTpdGpdTpdCpdGpdTpdTpdGpdGpdTpdGpdGpdCpdCpdCp-3'
Molecular Weight: 26177
Extinction Coefficient: 836200
Binding Conditions / Buffer
Phosphate-Buffered Saline (PBS) 1X with 4.5 mg/mL glucose [Acros], 5 mM MgCl2 [VWR]. 1 mg/mL bovine serum albumin (BSA). The buffers were filtered through a 0.22 um syringe filter.
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Tsogtbaatar, K., Sousa, D. A., Ferreira, D., Tevlek, A., Aydın, H. M., Çelik, E., & Rodrigues, L. (2021). In vitro selection of DNA aptamers against human osteosarcoma. Investigational New Drugs. doi:10.1007/s10637-021-01161-y
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