Ochratoxin A (H12) Aptamer Details
ID# 7757
Structure
Chemistry
DNA
Target
Ochratoxin A (OTA)
Category
Small Organic
Affinity (Kd)
96 nM
Length
66
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdGpdApdGpdGpdApdCpdGpdAp dApdGpdCpdGpdGpdApdApdCpdCpdGp dGpdGpdTpdGpdTpdGpdGpdGpdTpdGp dCpdCpdTpdTpdGpdApdTpdCpdCpdAp dGpdGpdGpdApdGpdTpdCpdTpdCpdApdGpdApdApdGpdApdCpdApdCpdGpdCp dCpdCpdGpdApdCpdAp-3'
Molecular Weight: 20569.34
Extinction Coefficient: 649700
Binding Conditions / Buffer
Binding Buffer: PBS 1X, 1 mM MgCl2, 0.01% Tween 20, pH 7.4.
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
L Barthelmebs et al. Enzyme-linked aptamer assays (ELAAs), based on a competition format for a rapid and sensitive detection of ochratoxin A in wine. Food Control 22(2011): 737-743
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