Nucleolin aptamer (AS1411) Aptamer Details

ID# 7675

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Structure

Chemistry

DNA

Target

Nucleolin

Sequence (3-char table code)

G A C U/T

5'-dGpdGpdTp dGpdGpdTp dGpdGpdTp dGpdGpdTp dTpdGpdTp dGpdGpdTp dGpdGpdTp dGpdGpdTp dGpdGp-3'

Molecular Weight: 8272.41

Extinction Coefficient: 250800

Binding Conditions / Buffer

For fluoresecent labeling, 400 nM aptamer were added to cell growth media for 1 hour at 37°C and 5% CO2. Dulbecco's phosphate-buffered saline was used to wash cells.

Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Kotula J, Sullenger B, et al. 2012. Aptamer-mediated delivery of splice-switching oligonucleotides to the nuclei of cancer cells. Nucleic Acid and Therapeutics 22.3:187-195. Li, L. et al. 2014. Aptamer photoregulation in vivo. PNAS 11.48: 17099-103.

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