NT-proBNP (N20a) Aptamer Details
ID# 10102
Structure
Chemistry
DNA
Target
N-terminal pro-peptide of B-type natriuretic peptide (NT-proBNP)
Category
Protein
Affinity (Kd)
2.89 nM
Length
72
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdCpdApdGpdGpdApdApdGpdApdCpdApdApdApdCpdApdGpdGpdTpdCpdGpdTpdApdGpdTpdGpdGpdApdApdApdCpdTpdGpdTpdCpdCpdApdCpdCpdGpdTpdApdGpdApdCpdCpdGpdGpdTpdTpdApdTpdCpdTpdApdGpdTpdGpdGpdTpdCpdTpdGpdTpdGpdGpdTpdGpdCpdTpdGpdTp-3'
Molecular Weight: 22383.5 g/mole
Extinction Coefficient: 705500
Binding Conditions / Buffer
140 mM NaCl and 5 mM MgCl2 in 20 mM tris(hydroxymethyl)aminomethane (Tris) HCl buffer (pH 7.4)
Binding Temp: 25°C
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Anirban Sinha, Priya Gopinathan, Yi-Da Chung, Hsin-Ying Lin, Kuang-Hsien Li, Hsi-Pin Ma, Po-Chiun Huang, Shu-Chu Shiesh, Gwo-Bin Lee, An integrated microfluidic platform to perform uninterrupted SELEX cycles to screen affinity reagents specific to cardiovascular biomarkers, Biosensors and Bioelectronics,
Volume 122, 2018, Pages 104-112, ISSN 0956-5663,
https://doi.org/10.1016/j.bios.2018.09.040.
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