Aptagen

NoPv1 Aptamer Details

ID# 8199
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Structure
NoPv1 structure
Chemistry
Peptide
Target
PvCesA2
Category
Protein
Affinity (Kd)
N/A nM
Length
8
Aptazyme
No

Sequence (3-char table code)

G A C U/T
NH₂-ArgLeuThrAlaGlnCysArgLeu-COOH
Molecular Weight: 959.53
Extinction Coefficient: 211167

Binding Conditions / Buffer

V. Vinifera cv. Pinot noir variety
Binding Temp: 22-25°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Colombo, M., Masiero, S., Rosa, S., Caporali, E., Toffolatti, S. L., Mizzotti, C., Tadini, L., Rossi, F., Pellegrino, S., Musetti, R., Velasco, R., Perazzolli, M., Vezzulli, S., & Pesaresi, P. (2020). Nopv1: A synthetic antimicrobial peptide aptamer targeting the causal agents of grapevine downy mildew and potato late blight. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-73027-x
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