Non-small Cell Lung Cancer (S6) Aptamer Details
ID# 7662
Structure
Chemistry
DNA
Target
Non-small Cell Lung Cancer
Category
Cells
Affinity (Kd)
28.2 nM
Length
85
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdCpdGpdCpdTpdCpdGpdGpdApdTpdGpdCpdCpdApdCpdTpdApdCpdApdGpdGpdTpdGpdGpdCpdCpdApdGpdTpdCpdApdCpdTpdCpdApdApdTpdTpdGpdGpdGpdTpdGpdTpdApdGpdGpdGpdGpdTpdGpdGpdGpdGpdApdTpdTpdGpdTpdGpdGpdGpdTpdTpdGpdCpdTpdCpdApdTpdGpdGpdApdCpdGpdTpdGpdCpdTpdGpdGpdTpdGpdApdCp-3'
Molecular Weight: 26491.17
Extinction Coefficient: 809200
Binding Conditions / Buffer
Wash Buffer (5 mmol MgCl2 and 4.5 g glucose into 1 L of Dulbeccoƒ??s PBS without calcium and magnesium) The binding buffer was prepared by adding 1 mg/mL bovine serum albumin (BSA) and 0.1 mg/mL yeast tRNA to washing buffer
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Zhao et al. "Recognition of subtype non-small cell lung cancer by DNA aptamers selected from living cells." Analyst 134(2009):1808-1814.
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