NF-kappa B (Aptamer 3) Aptamer Details
ID# 7544
Structure
Chemistry
RNA
Target
NF-kappa B
Category
Protein
Affinity (Kd)
1 nM
Length
97
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprUprAprUprCprCprUprCprGprAprGprCprAprUprAprAprGprAprAprAprCprAprAprGprAprUprAprGprAprUprCprCprUprGprAprAprAprCprUprGprUprUprUprUprAprAprGprGprUprUprGprGprCprCprGprAprUprCprUprUprCprUprGprCprUprCprGprAprGprAprAprUprGprCprAprUprGprAprAprGprCprGprUprUprCprCprAprUprAprUprUprUprUprUp-3'
Molecular Weight: 31122.71
Extinction Coefficient: 981700
Binding Conditions / Buffer
Buffer A (20 mM HEPES (pH 7.5), 0.4 M NaCl, 2 mM EDTA, 5% v/v glycerol, 1 mM DTT, 0.5 mM PMSF, 1 ug/mL pepstatin, 1 ug/mL leupeptin) Buffer B (20 mM HEPES (pH 7.5), 2 mM EDTA, 5% v/v glycerol, 1 mM DTT, 0.5 mM PMSF, 1 ug/mL pepstatin, 1 ug/mL leupeptin) Selection Buffer (10 mM HEPES (pH 7.5) 0.1 M NaCl, 1 mM DTT)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Lebriska and Maher. "Selection and Characterization of an RNA Decoy for Transcription Factor NF- B." Journal of Biochemistry, 38(1999): 3168-3174.
Request a Quote
Have your aptamer oligo synthesized by Aptagen.
