nDNA-547 Aptamer Details
ID# 8141
Structure
Chemistry
DNA
Target
nano-sized DNA
Category
Small Organic
Affinity (Kd)
363 ± 135 nM nM
Length
72
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5’-dApdCpdCpdApdCpdApdApdCpdGpdGpdTpdTpdTpdCpdCpdCpdApdGpdGpdApdCpdGpdGpdApdTpdGpdCpdTpdTpdCpdCpdGpdTpdApdApdTpdGpdApdCpdGpdCpdTpdTpdCpdCpdCpdApdTpdTpdApdCpdCpdCpdGpdTpdCpdCpdApdApdTpdCpdApdApdCpdTpdCpdApdApdCpdTpdTpdCp-3’
Molecular Weight: 21887.2
Extinction Coefficient: 666800
Binding Conditions / Buffer
7 M urea, 10 mM EDTA, 300 mM NaOAc, pH 5.2
Binding Temp: 26°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Ogawa, A., & Itoh, Y. (2020). In Vitro Selection of RNA Aptamers Binding to Nanosized DNA for Constructing Artificial Riboswitches. ACS Synthetic Biology. doi:10.1021/acssynbio.0c00384
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