NC3S Aptamer Details
ID# 8079
Structure
Chemistry
DNA
Target
N‑Cadherin
Category
Protein
Affinity (Kd)
20.08 ± 0.72 nM
Length
47
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5’-dTpdTpdGpdCpdApdCpdTpdApdTpdGpdTpdTpdTpdTpdApdGpdCpdTpdApdGpdGpdGpdTpdTpdCpdCpdCpdTpdCpdCpdGpdGpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdAp-3’
Molecular Weight: 14499.4
Extinction Coefficient: 454400
Binding Conditions / Buffer
4.5 g L−1 glucose and 5 mM MgCl2 in Dulbecco’s PBS without calcium and magnesium, yeast tRNA and BSA
Binding Temp: 4, 25, 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Gao T , Ding P , Li W , Wang Z , Lin Q , Pei R . Isolation of DNA aptamers targeting N-cadherin and high-efficiency capture of circulating tumor cells by using dual aptamers. Nanoscale. 2020 Nov 19;12(44):22574-22585. doi: 10.1039/d0nr06180h. PMID: 33174555.
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