Nanobioprobe mediated DNA aptamer Aptamer Details
ID# 7894
Structure
Chemistry
DNA
Target
Trinitrotoluene (TNT)
Category
Other
Affinity (Kd)
100 nM
Length
88
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdGpdGpdGpdApdCpdApdGpdTpdCpdGpdApdTpdGpdGpdGpdApdCpdGpdGpdCpdApdApdApdCpdGpdGpdApdCpdCpdApdGpdTpdGpdTpdGpdTpdGpdGpdCpdGpdGpdTpdApdGpdApdTpdApdGpdTpdApdApdGpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp
Molecular Weight: 27415.9
Extinction Coefficient: 890400
Binding Conditions / Buffer
10 mM Tris/HCl buffer, pH 7.6, 150 mM NaCl, 5 mM MgCl2
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Priyanka, M. S., et al. "Nanobioprobe mediated DNA aptamers for explosive detection." Chem. Commun., 2014, 50, 1080 - 1082.
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