Nampt binding aptamer (NBA) Aptamer Details

ID# 7775

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Structure

Chemistry

DNA

Target

Nampt/Nicotinamide phosphoribosyltransferase

Sequence (3-char table code)

G A C U/T

5'-dApdTpdAp dCpdCpdAp dGpdCpdTp dTpdApdTp dTpdCpdAp dApdTpdTp dGpdGpdGp dCpdApdGp dGpdApdCp dApdGpdGp dTpdGpdTp dCpdGpdGp dCpdTpdTp dGpdApdTp dApdGpdGp dCpdTpdGp dGpdGpdGp dTpdGpdTp dGpdTpdGp dTpdApdGp dApdTpdAp dGpdTpdAp dApdGpdTp dGpdCpdAp dApdTpdCp dTp-3'

Molecular Weight: 23679.45

Extinction Coefficient: 748600

Binding Conditions / Buffer

Binding Buffer: 100 mM NaCl, 25 mM Tris-HCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, and 0.02% Tween-20, pH 7.6.

Binding Temp: RT°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Park, J.-W., Tatavarty, R., Kim, D. W., Jung, H.-T., & Gu, M. B. (2012). Immobilization-free screening of aptamers assisted by graphene oxide. Chem. Commun., 48(15), 2071–2073. doi:10.1039/c2cc16473f SJ Lee et al. Sensitive detection of adipokines for early diagnosis of type 2 diabetes using enzyme-linked antibody-aptamer sandwich (ELAAS) assays. Sensors Actuat. B-Chem.(2012): 243-248.

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