NADH Aptamer Details
ID# 7667
Structure
Chemistry
RNA
Target
NADH
Category
Small Organic
Affinity (Kd)
37 nM
Length
49
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprAprAprCprCprCprAprAprCprUprAprGprGprCprGprUprUprUprGprAprGprGprGprGprAprUprUprCprGprGprCprCprAprCprGprGprUprAprAprCprAprAprCprCprCprCprUprCp-3'
Molecular Weight: 15748.56
Extinction Coefficient: 474700
Binding Conditions / Buffer
Selection Buffer (20 mM HEPES (pH 7.5), 300 mM KC1, and 5 mM MgC12) at room temperature.
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Lauhon and Szostak. "RNA Aptamers That Bind Flavin and Nicotinamide Redox Cofactors." Journal of the American Chemical Society, 117(1994): 1246-1257.
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