Aptagen

NaA43 Aptamer Details

ID# 7955
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Structure
NaA43 structure
Chemistry
DNA
Target
Sodium Ion
Category
Other
Affinity (Kd)
39.1 mM
Length
61
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dGpdCpdGpdGpdCpdGpdGpdTpdApdCpdCpdApdGpdGpdTpdCpdApdApdApdGpdGpdTpdGpdGpdGpdTpdGpdApdGpdGpdGpdGpdApdCpdGpdCpdCpdApdApdGpdApdGpdTpdCpdCpdCpdCpdGpdCpdGpdGpdTpdTpdApdCpdApdTpdApdGpdApdGp-3'
Molecular Weight: 19035.35
Extinction Coefficient: 602700

Binding Conditions / Buffer

Dulbeccoƒ??s modification of Eagleƒ??s medium (DMEM) supplemented with 10% Fetal Bovine Serum (FBS), 100 U/mL penicillin, and 100 µg/mL streptomycin,
Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Torabi, S., Wu, P., McGhee, C., Chen, L., Hwang, K., Zheng, N., Cheng, J., & Lu, Y. (2015) In Vitro Selection of a Sodium-Specific DNAzyme and its Application in Intracellular Sensing. PNAS 112, (19):5903-5908
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