Mycoplasma bovis detection aptamer (WKB-14) Aptamer Details
ID# 7899
Structure
Chemistry
DNA
Target
P48 of M. Bovis
Category
Protein
Affinity (Kd)
15.61 nM
Length
80
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dGpdCpdTpdGpdCpdApdApdTpdApdCpdTpdCpdApdTpdGpdGpdApdCpdApdGpdGpdTpdTpdGpdCpdGpdApdApdApdGpdApdCpdApdApdCpdGpdApdApdTpdGpdCpdTpdTpdTpdGpdCpdCpdTpdGpdCpdCpdApdTpdApdApdTpdTpdTpdGpdCpdGpdTpdCpdTpdGpdGpdApdGpdTpdApdCpdGpdApdCpdCpdCpdTpdGpdApdAp
Molecular Weight: 24687.08
Extinction Coefficient: 769800
Binding Conditions / Buffer
Added to wells of plate coated with P48 protein solution (P48 protein dissolved in 100 uL of 0.05 carbonate-bicarbonate buffer)
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Fu, P., et. al. Enzyme linked aptamer assay: based on a competition format for sensitive detection of antibodies to Mycoplasma bovis in serum.
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