MT32 protein of Apple Stem Pitting Virus (ASPV) Aptamer Details
ID# 7560
Structure
Chemistry
DNA
Target
MT32 coat protein
Category
Protein
Affinity (Kd)
55 nM
Length
40
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdGpdGpdTpdGpdGpdTpdGpdGpdGpdTpdTpdCpdTpdTpdTpdTpdTpdGpdTpdGpdGpdTpdApdTpdTpdGpdGpdTpdGpdTpdGpdGpdGpdGpdGpdGpdCpdAp-3'
Molecular Weight: 12619.23
Extinction Coefficient: 380300
Binding Conditions / Buffer
Selection Buffer: PBS-T (10 mM Na2HPO4, 2 mM KH2PO4, 2.7 mM KCl, 137 mM NaCl, and 0.05%Triton-X; pH 7.5) with added 0.1 μg/ml poly-dIdC and 1 μg/ml BSA.
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Balogh, et al. "Selection and versatile application of virus-specific aptamers." FASEB Journal, 24(2010): 4187-4195.
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