MRP1Apt (3) Aptamer Details

ID# 8998

Back to Search Results

Structure

Chemistry

RNA

Target

MRP1

Sequence (3-char table code)

G A C U/T

rGprGprGprAprGprAprAprAprAprAprGprAprAprUprAprGprUprCprAprAprCprAprAprAprUprCprGprUprUprUprGprGprGprGprCprGprAprCprUprUprCprUprCprCprUprUprCprCprUprUprUprCprUprCprCprCprUprUprCprUprCprCprCp

Molecular Weight: 19015 g/mole

Extinction Coefficient: 563500

Binding Conditions / Buffer

The pre-cleared RNA pool was incubated with the bead-coupled MRP1 peptide and washed three times with washing buffer that contained 150 mmol/l NaCl, 2 mmol/l CaCl2, 20 mmol/l HEPES (pH 7.4), so as to remove the non-interacting RNA species. The MRP-1 peptide-binding RNA species were extracted after 30 minutes of incubation through phenol:chloroform:isoamyl alcohol (25:24:1), followed by reverse transcription and PCR amplification

Binding Temp: 37°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Soldevilla MM, Villanueva H, Casares N, Lasarte JJ, Bendandi M, Inoges S, López-Díaz de Cerio A, Pastor F. MRP1-CD28 bi-specific oligonucleotide aptamers: target costimulation to drug-resistant melanoma cancer stem cells. Oncotarget. 2016 Apr 26;7(17):23182-96. doi: 10.18632/oncotarget.8095. PMID: 26992239; PMCID: PMC5029619.

Request a Quote Have your aptamer oligo synthesized by Aptagen.