Microcystin (RC6) Aptamer Details

ID# 7827

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Structure

Chemistry

DNA

Target

Microcystin

Sequence (3-char table code)

G A C U/T

5'-dCpdApdCpdGpdCpdApdApdCpdApdApdCpdApdCpdApdApdCpdApdTpdGpdCpdCpdCpdApdGpdCpdGpdCpdCpdTpdGpdGpdApdApdCpdApdTpdApdTpdCpdCpdTpdApdTpdGpdApdGpdTpdTpdApdGpdTpdCpdCpdGpdCpdCpdCpdApdCpdAp-3'

Molecular Weight: 18280.89

Extinction Coefficient: 573300

Binding Conditions / Buffer

Binding Buffer: 50 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM MgCl2

Binding Temp: 25°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Ng A, Zourob M, et al. (2012) Selection, characterization and biosensing application of high affinity congener-specific microcystin-targeting aptamers. Environ. Sci. Technol. 46: 10697-10703.

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