Microcystin-LR (AN6) Aptamer Details
ID# 7828
Structure
Chemistry
DNA
Target
Microcystin-LR
Category
Small Organic
Affinity (Kd)
50 nM
Length
60
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdCpdGpdCpdCpdApdApdApdCpdApdGpdGpdApdCpdCpdApdCpdCpdApdTpdGpdApdCpdApdApdTpdTpdApdCpdCpdCpdApdTpdApdCpdCpdApdCpdCpdTpdCpdApdTpdTpdApdTpdGpdCpdCpdCpdCpdApdTpdCpdTpdCpdCpdGpdCp-3'
Molecular Weight: 18167.79
Extinction Coefficient: 557700
Binding Conditions / Buffer
Binding Buffer: 50 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM MgCl2
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Ng A, Zourob M, et al. (2012) Selection, characterization and biosensing application of high affinity congener-specific microcystin-targeting aptamers. Environ. Sci. Technol. 46: 10697-10703.
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