mHtt2.2.47 Aptamer Details
ID# 8221
Structure
Chemistry
RNA
Target
51Q-htt
Category
Protein
Affinity (Kd)
388.3 nM
Length
75
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprAprGprAprGprAprGprAprCprAprGprUprCprUprGprCprCprCprCprGprAprUprUprAprAprAprAprUprAprGprCprAprCprAprGprCprAprAprCprCprAprAprCprGprCprCprCprCprCprCprCprAprAprGprCprAprAprCprGprUprCprAprAprCprUprCprCprAprGprAprAp-3'
Molecular Weight: 24184.54
Extinction Coefficient:
Binding Conditions / Buffer
2, 3, or 4 mmol/l MgCl2 in 50 mmol/l phosphate buffer, 150 mmol/l NaCl, pH 7.4.
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Rajeev K Chaudhary, Kinjal A Patel, Milan K Patel, Radha H Joshi, Ipsita Roy, Inhibition of Aggregation of Mutant Huntingtin by Nucleic Acid Aptamers In Vitro and in a Yeast Model of Huntington's Disease, Molecular Therapy, Volume 23, Issue 12, 2015, Pages 1912-1926, ISSN 1525-0016.
Request a Quote
Have your aptamer oligo synthesized by Aptagen.
