Methylenedianiline (M1) Aptamer Details
ID# 7496
Structure
Chemistry
RNA
Target
Methylenedianiline
Category
Small Organic
Affinity (Kd)
450 nM
Length
87
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprAprCprAprAprGprAprAprUprAprAprAprCprGprCprUprCprAprAprCprUprGprCprGprAprUprCprAprGprGprGprGprUprAprAprAprUprUprUprCprCprGprCprGprCprAprGprGprCprUprCprCprAprCprGprCprCprGprCprUprUprCprGprAprCprAprGprGprAprGprGprCprUprCprAprCprAprAprCprAprGprGprCp-3'
Molecular Weight: 28102.08
Extinction Coefficient: 852400
Binding Conditions / Buffer
Binding buffer: 250 mM NaCl, 50 mM Trisƒ??HCl, 5 mM MgCl2
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Brockstedt, U., et al. "In vitro evolution of RNA aptamers recognizing carcinogenic aromatic amines." Biochemical and Biophysical Research Communications, 313 (2004): 1004-1008.
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