Mercury II (Hg2+) Aptamer Details
ID# 7707
Structure
Chemistry
DNA
Target
Mercury II (Hg2+)
Category
Other
Affinity (Kd)
40 nM
Length
22
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dTpdTpdCpdTpdTpdTpdCpdTpdTpdCpdCpdCpdCpdTpdTpdGpdTpdTpdTpdGpdTpdTp-3'
Molecular Weight: 6590.34
Extinction Coefficient: 178600
Binding Conditions / Buffer
3-(N-morpholino)propanesulfonic acid (10mM, pH 7.0, NaCl (25mM), NaNO3 (500mM), and ethylenediamine (0.1 mM)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
A. Ono and H. Togashi. "Highly selective oligonucleotide-based sensor for mercury(II) in aqueous solutions." Angew. Chem. Int. Ed. 43(2004):4300-4302.
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