Membrane-Bound IgM (TD05.17) Aptamer Details
ID# 7717
Structure
Chemistry
Chimeric
Target
Membrane-bound IgM (mIgM )/ B-cell receptor (BCR )/ Leukemia / Lymphoma
Category
Protein
Affinity (Kd)
43 nM
Length
37
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdGpdGpdApdGpdGpdApdTpdApdGp dTpdTpdCpdGpdGpdTpdGpdGpdCpdTp dGpdTpdTpdCpdApdGpdGpdGpdTpdCp dTpdCpdCpbTnbCnbCnbTn-3'
Molecular Weight: 10256.7
Extinction Coefficient: 349100
Binding Conditions / Buffer
Binding Buffer: RPMI 1640, 4.5 g/L glucose, 5 mM MgCl2 in vivo (20 mM MgCl2 in vitro), 100 mg/L tRNA and 100 mg/L BSA.
Binding Temp: 4°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
R Mallikaratchy et al. "A multivalent DNA aptamer specific for the B-cell receptor on human lymphoma and leukemia." Nucleic Acids Res. 39(2011): 2458-2469.
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