MBMI-01c Aptamer Details
ID# 8222
Structure
Chemistry
DNA
Target
α-conotoxin MI (CTX-MI)
Category
Peptide
Affinity (Kd)
0.524 nM
Length
80
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdTpdTpdGpdGpdCpdApdCpdTpdCpdCpdApdCpdGpdCpdApdTpdApdGpdGpdTpdTpdTpdGpdGpdGpdGpdApdTpdGpdGpdGpdCpdApdApdCpdGpdGpdTpdApdApdApdApdApdGpdGpdGpdTpdCpdApdApdApdApdGpdGpdCpdTpdTpdTpdTpdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTpdApdCpdCpdGpdTpdGpdApdAp-3'
Molecular Weight:
Extinction Coefficient:
Binding Conditions / Buffer
The selection Buffer (SB) (0.9 mM CaCl2, 2.7 mM KCl, 1.5 mM KH2PO4,
0.6 mM MgCl2·6H2O, 0.1 M NaCl and 20 mM Na2HPO4, pH = 7.4) was purchased from
Tiandz (Beijing, China). The SB was used for aptamer screening, the BLI assay, and the
performance evaluation of the aptasensor.
Binding Temp: 20°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Guo, H.; Deng, B.; Zhao, L.; Gao, Y.; Zhang, X.; Yang, C.; Zou, B.; Chen, H.; Sun, M.; Wang, L.; et al.
Programmed Aptamer Screening, Characterization, and Rapid Detection for α-Conotoxin MI. Toxins 2022, 14, 706. https://doi.org/10.3390/toxins14100706
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