mApoc46 Aptamer Details
ID# 9541
Structure
Chemistry
DNA
Target
Patient-derived high-grade serous ovarian cancer (HG-SOC) cells
Category
Cells
Affinity (Kd)
150 ± 50 nM for pSOC cells nM
Length
59 nucleotides
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dGpdTpdGpdCpdTpdGpdGpdApdTpdGpdTpdCpdApdCpdCpdTpdCpdCpdApdCpdTpdCpdApdCpdCpdApdCpdCpdTpdCpdTpdTpdCpdTpdCpdCpdGpdCpdTpdCpdCpdTpdCpdGpdTpdCpdApdTpdGpdApdCpdApdCpdApdTpdCpdCpdApdGp
Molecular Weight: ≈ 18–19 kDa
Extinction Coefficient:
Binding Conditions / Buffer
Incubate 100–150 nM FAM-aptamer with 1×10⁵ target cells in DPBS (Ca²⁺/Mg²⁺, pH 7.4) + 0.05 g/L BSA + 2 µg/mL salmon sperm DNA at 4 °C for 20–30 min, then wash with DPBS.
Binding Temp: 4 °C for cell binding; 25 °C for SPR; various temps for stability tests at 37 °C
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Wang, J., Fang, X., Zhang, C., Ji, H., Pang, Q., Li, X., Luo, Z., Wu, Q., & Zhang, L. (2021). Development of aptamer-based molecular tools for rapid intraoperative diagnosis and in vivo imaging of serous ovarian cancer. ACS Applied Materials & Interfaces, 13(19), 22048–22061. https://doi.org/10.1021/acsami.1c02072
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