Aptagen

Malachite Green (MG-4) Aptamer Details

ID# 7481
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Structure
Malachite Green (MG-4) structure
Chemistry
RNA
Target
Malchite Green
Category
Small Organic
Affinity (Kd)
1 µM
Length
38
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-rGprGprAprUprCprCprCprGprAprCprUprGprGprCprGprAprGprAprGprCprCprAprGprGprUprAprAprCprGprAprAprUprGprGprAprUprCprCp-3'
Molecular Weight: 12300.52
Extinction Coefficient: 377500

Binding Conditions / Buffer

Selection buffer: 0.1 M KCl, 5 mM MgCl2, 10 mM Na-HEPES (pH 7.4)
Binding Temp:

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Grate, Dilara and Wilson, Charles. "Laser-mediated, site-specific inactivation of RNA transcripts." PNAS, 96 (1999): 6131-6136
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