M64RA Aptamer Details
ID# 7669
Structure
Chemistry
DNA
Target
MPT64 Protein
Category
Protein
Affinity (Kd)
N/A nM
Length
35
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'- dTpdGpdGpdGpdApdGpdCpdTpdGpdAp dTpdGpdTpdCpdGpdCpdApdTpdGpdGp dGpdTpdTpdTpdTpdGpdApdTpdCpdAp dCpdApdTpdGpdAp-3'
Molecular Weight: 10873.13
Extinction Coefficient: 339000
Binding Conditions / Buffer
SHCMK binding buffer: 20 mM HEPES, pH 7.35, 120 mM KCL, 1 mM CaCl2, and 1 mM MgCl2
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Qin, L., et al.(2009). "The selection and application of ssDNA aptamers against MPT64 protein in Mycobacterium tuberculosis." Clin Chem Lab Med, 47(4), 405-411. doi: 10.1515/CCLM.2009.097
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