M64CA Aptamer Details

ID# 7668

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Structure

Chemistry

DNA

Target

MPT64 Protein

Sequence (3-char table code)

G A C U/T

5'- dTpdTpdCpdGpdGpdGpdApdApdTpdGp dApdTpdTpdApdTpdCpdApdApdApdTp dTpdTpdApdTpdGpdCpdCpdCpdTpdCp dTpdGpdApdTp-3'

Molecular Weight: 10421.87

Extinction Coefficient: 326100

Binding Conditions / Buffer

SHCMK binding buffer: 20 mM HEPES, pH 7.35, 120 mM KCL, 1 mM CaCl2, and 1 mM MgCl2

Binding Temp: RT°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Qin, L., et al.(2009). "The selection and application of ssDNA aptamers against MPT64 protein in Mycobacterium tuberculosis." Clin Chem Lab Med, 47(4), 405-411. doi: 10.1515/CCLM.2009.097

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