M3 Aptamer Aptamer Details
ID# 8007
Structure
Chemistry
DNA
Target
MDA-MB-231 cells
Category
Cells
Affinity (Kd)
45.6 nM
Length
85
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'- dApdApdGp dGpdApdGp dCpdApdGp dCpdGpdTp dGpdGpdAp dGpdGpdAp dTpdApdTp dApdCpdAp dCpdCpdGp dTpdCpdAp dTpdCpdAp dGpdApdGp dGpdGpdAp dGpdCpdAp dTpdCpdTp dCpdTpdAp dGpdTpdCp dApdGpdGp dApdCpdTp dGpdTpdGp dApdTpdGp dApdApdTp dTpdApdGp dGpdGpdTp dGpdTpdGp dTpdCpdGp dTpdCpdGp dTpdGpdGp dTp -3'
Molecular Weight: 26524.2
Extinction Coefficient: 848300
Binding Conditions / Buffer
PBS containing 5 mM MgCl2, 4.5 gL-1 glucose, 1 mg mL^-1 BSA, and 0.1 mg mL salmon sperm DNA
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Li, W.M.; Zhou, L.L.; Zheng, M.; Fang, J. Selection of Metastatic Breast Cancer Cell-Specific Aptamers for the Capture of CTCs with a Metastatic Phenotype by Cell-SELEX. Mol. Ther. Nucleic Acids 2018, 12, 707-717.
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