Lysozyme (Clone 1) Aptamer Details
ID# 7823
Structure
Chemistry
DNA
Target
Hen egg white lysozyme
Category
Protein
Affinity (Kd)
29-31 nM
Length
80
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdGpdApdApdTpdGpdGpdApdTpdCpdCpdApdCpdApdTpdCpdTpdApdCpdGpdApdApdTpdTpdCpdApdTpdCpdApdGpdGpdGpdCpdTpdApdApdApdGpdApdGp dTpdGpdCpdApdGpdApdGpdTpdTpdApdCpdTpdTpdApdGpdTpdTpdCpdApdCpdTpdGpdCpdApdGpdApdCpdTpdTpdGpdApdCpdGpdApdApdGpdCpdTpdTp -3'
Molecular Weight: 24750.16
Extinction Coefficient: 788000
Binding Conditions / Buffer
Tris Buffer: 20 mM Tris pH 7.6, 100 mM NaCl, 5 mM MgCl2
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Cox JC and Ellington AD. (2001) Automated selection of anti-protein aptamers. Bioorg. Med. Chem. 9: 2525 - 2531.
Kirby R, et al. (2004) Aptamer-based sensor arrays for the detection and quantitation of proteins. Anal. Chem. 76: 4066 - 4075.
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