Lysozyme (Apt 80) Aptamer Details
ID# 7821
Structure
Chemistry
DNA
Target
Hen egg white lysozyme and recombinant human lysozyme
Category
Protein
Affinity (Kd)
2.8 nM
Length
85
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdGpdCpdApdGp dCpdApdCpdApdGp dApdGpdGpdTpdCp dApdGpdApdTpdGp dGpdCpdApdGpdGp dTpdApdApdGpdCp dApdGpdGpdCpdGp dGpdCpdTpdCpdAp dCpdApdApdApdAp dCpdCpdApdTpdTp dCpdGpdCpdApdTp dGpdCpdGpdGpdCp dCpdCpdTpdApdTp dGpdCpdGpdTpdGp dCpdTpdApdCpdCp dGpdTpdGpdApdAp -3'
Molecular Weight: 24742.06
Extinction Coefficient: 777200
Binding Conditions / Buffer
Binding Buffer: 25 mM trizma base, 192 mM glycine, and 5 mM K2HPO4, pH 8.3.
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Tran DT, et al. (2010) Selection and characterization of DNA aptamers for egg white lysozyme. Molecules 15: 1127 - 1140.
Zou M et al. (2012) The homogeneous fluorescence anisotropic sensing of salivary lysozyme using the 6-carboxyfluorescein-labeled DNA aptamer. Biosens. Bioelectron. 32: 148-154.
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