Lysozyme Aptamer Details
ID# 7563
Structure
Chemistry
RNA
Target
Lysozyme
Category
Protein
Affinity (Kd)
31 nM
Length
79
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprAprUprGprGprAprUprCprCprAprCprAprUprCprUprAprCprGprAprAprUprUprCprAprUprCprAprGprGprGprCprUprAprAprAprGprAprGp-T-rGprCprAprGprAprGprUprUprAprCprUprUprAprGprUprUprCprAprCprUprGprCprAprGprAprCprUprUprGprAprCprGprAprAprGprCprUprUp-3'
Molecular Weight: 25443.39
Extinction Coefficient: 800700
Binding Conditions / Buffer
Selection Buffer (20 mM Tris (pH 7.5) 100 mM NaCl,5 mM MgCl2)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Cox and Ellington. "Automated Selection of Anti-Protein Aptamers." Bioorganic & Medicinal Chemsitry, 9 (2001): 2525-2531/
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